Proteins detected in an SHR serum. Spots 1, 50, 85, 117 and 211 were significantly altered > 자유게시판

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Proteins detected in an SHR serum. Spots 1, 50, 85, 117 and 211 were significantly altered in levels when image analysis comparing the expression of 2-DE protein spots of SDR serum samples with those of the SHR. Acid side of the gel is to the left and relative molecular mass declines from the top.Aminudin et al. Proteome Science 2012, 10:17 http://www.proteomesci.com/content/10/1/Page 3 ofwere corrected for false significant results using the method of Benjamini and Hochberg [8] (Figure 2). Using the serum proteins expressed in the sera of normotensive SDR as a standard reference, the levels of two proteins were found to be significantly lowered (spotsand 117) and three proteins (spots 50, 85 and 211) were apparently enhanced in the sera of the SHR. However, only spots 1, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/744568 50 and 85 maintained to be significantly altered in levels when the serum 2-DE profiles of SHR that were treated with captopril were compared those ofFigure 2 Relative expression of protein spots of interest in sera of SDR, SHR and captopril-treated SHR. The percentage of volume contribution was determined using the Image Master 2D Platinum Software 7.0. Relative volumes of spots 1, 50, 85, 117 4-Bromo-5-nitro-1H-indazole and 211 were significantly different when comparison was made between SHR and SDR. However, only spots 1, 50 and 85 were significantly different when the 2-DE images of captopriltreated SHR were compared to those obtained from SDR. Y axis of histogram indicates percentage of volume contribution. Asterisks denote significantly different values relative to the SDR (p< 0.00233).Aminudin et al. Proteome Science 2012, 10:17 http://www.proteomesci.com/content/10/1/Page 4 ofthe normotensive SDR (Figure 2). The expression of spots 117 and 211 was apparently no longer significantly different and appeared to be normalised. Cropped images of the five serum protein spots in 2-DE PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/3081428 gels of control and experimental rats are shown in Figure 3.Identification of differentially-expressed rat serum proteinsThe five differentially expressed protein spots were confidently identified by mass spectrometry using the 4800 Plus MALDI TOF/TOF analyzer and MASCOT database search (Table 2). They were retinol binding protein 4 (RBP4; spot 1), complement C3 (C3; spot 85), albumin 2-Chloro-5,6-dihydro-7H-cyclopenta[b]pyridin-7-one (ALB; spot 50), alpha1 macroglobulin (A1MG; spot 117) and alpha 1 antiproteinase (A1AP; spot 211). The albumin spot was apparently resolved in the region of around 19.9 kDa in the 2-DE serum protein profiles, suggesting that it was a fragment of the protein (ALBf).Discussion In the present study, we firstly demonstrated a significant hypotensive effect of captopril following a 10-day treatment. This acute duration treatment showed a 20 hypotensive response, whereby the baseline blood pressure of 191 mmHg was reduced to 151 mmHg (Table 1). When the sera of SHR were subjected to 2-DE andFigure 3 Representative cropped images of selective protein spots of interest in rat serum. Panels A, B and C refer to the protein spots detected in the sera of SDR, SHR and Captopril treated SHR (C-SHR), respectively.compared to similar profiles generated from those of SDR, the levels of five protein spots were found to be significantly altered. The spots were subsequently identified as those of the RBP4, C3, a 19.9 kDa fragment of ALB, A1MG and A1AT (Table 2). All proteins have been previously identified to have some association with hypertension. The levels the proteins were earlier reported to be altered in the SHR as well as patients with hypertensi.